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sgscramble bfp  (Addgene inc)


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  • 93

    Structured Review

    Addgene inc sgscramble bfp
    Sgscramble Bfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgscramble+bfp/pU6-sgRNA+EF1Alpha-puro-T2A-BFP%2EControl+(Plasmid+%23128749)/pmc11145263-121-81-82
    Average 93 stars, based on 2 article reviews
    sgscramble bfp - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Tau fibrils induce nanoscale membrane damage and nucleate cytosolic tau at lysosomes
    Article Snippet: All cells were maintained in a copper-lined Heracell VIOS 160i tissue culture incubator (ThermoFischer catalog: 51033574) at 37 °C and 5% CO2 and checked for Mycoplasma contamination. .. To generate K18.tau-mClover, TMEM192–RFP, pHLARE, CHMP2B–HALO, CHMP2B intron5 –HALO, CHMP4B–HALO, Gal3–GFP, dCas9–KRAB, sgScramble, and sgCHMP6 lentiviruses, 2E6 HEK293T cells were seeded into 10 cm plates and transfected the next day with 45 μL Mirus LT1 transfection reagent (MIR2300) added to a mixture of 5 μg each (15 μg total) of plasmids VSV-G (addgene: 8454), R8.74 (addgene: 22036), and one of the following: K18.tau-mClover (addgene: 133058), TMEM192–RFP (addgene: 134631), pHLARE (addgene: 164478), CHMP2B–HALO, CHMP2B–HALO, CHMP2B intron5 –HALO (this study), Gal3–GFP (addgene: 62734), sgScramble-BFP (addgene: 128749), dCas9-KRAB, or sgCHMP6 (Martin Kampmann lab) in 1.5 mL Optimem (ThermoFischer catalog: 31985062) according to the manufacturer’s recommendations. .. The supernatant containing viruses was obtained 3 d posttransfection, clarified by centrifugation at 2,500 rpm for 2.5 min and concentrated 10-fold using Lenti-X concentrator (Takara Bio catalog: 631231).



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    93
    Addgene inc sgscramble bfp
    Sgscramble Bfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgscramble+bfp/pU6-sgRNA+EF1Alpha-puro-T2A-BFP%2EControl+(Plasmid+%23128749)/pmc11145263-121-81-82
    Average 93 stars, based on 1 article reviews
    sgscramble bfp - by Bioz Stars, 2026-10
    93/100 stars
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